GenBit
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ISTA Biotechnology Trait Detection Workshop
The International Seed Testing Association (ISTA), Federal Budget State Enterprise "The Russian Agricultural Center" ("Rosselhoztsentr") и "GenBit" LLC conducted the training workshop on GMO testing in seed and plant material.
The Workshop Programme included both theoretical and practical courses.
Teachers - members of the ISTA GMO Committee and GenBit LLC.
Target audience - representatives of the Russian public and private entities interested and involved in plant GMO testing.
The training workshop was held on August 28-31, 2018 г.
Working language - English.
You can launch questions to:
ISTA
FBSE "Rosselhoztsentr"
"GenBit" LLC
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Технопарк "Слава"
117246 Российская Федерация, Москва,
Научный проезд, д. 20, строение 2
More Info
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"Slava" technopark
117246 Russian FederationMoscow, Nauchny proezd 20, build. 2
Classrooms
Laboratory
Biotechnology Trait Detection Workshop
August 27-31, 2018
Moscow, Russia
Organized by:International Seed Testing Association
GMO Technical Committee
FBSE "The Russian Agricultural Center"
GenBit LLC
Lecturers and organizers:
Enrico Noli, PhD.
University of Bologna
ISTA GMO Committee Chair
enrico.noli@unibo.it
Bruno Zaccomer, PhD
Monsanto
Peyrehorade, France
bruno.zaccomer@monsanto.com
Kirk Remund, Science Fellow
Monsanto
St. Louis, MO, USA
kirk.m.remund@monsanto.com
Alexander Golikov, Ph.D.
GenBit LLC
Moscow, Russian Federation
golikov@genbitgroup.com
Maxim Nikitin, Ph.D.
GenBit LLC
Moscow, Russian Federation
nikitin@genbitgroup.com
Pavel Frantsuzov, Ph.D.
GenBit LLC
Moscow, Russian Federation
frantsuzov@genbitgroup.com
programme
Monday, 27 August 2018
- 18:00 Welcome Dinner
Tuesday, 28 August 2018
- 08:30 Opening and introductions
- 09:00 Introduction to GMO testing & workshop overview
- 10:00 Coffee break
testing plans
- 10:30 Theory: Testing plans I. Basic statistical concepts
- 11:15 Qualitative testing plans – Introduction to SeedCalc
- 12:00 Lunch
- 13:00 Theory: Testing plans II. Quantitative testing plans
- 14:30 Computer exercises
- 16:00 End of the day 1
Wednesday, 29 August 2018
from seed to dna
- 08:30 Theory: Sample Preparation: I. DNA Extractions
- 09:30 Introduction to the Hands-On laboratory procedure and design
- 10:00 Coffee break
- 10:30 Hands-On Laboratory: DNA extraction
- 12:00 Lunch
- 13:00 Theory: Sample Preparation: II. DNA quantitation, normalization, and sample tracking
- 14:30 Hands-On Laboratory: DNA visualization, quantitation and normalization
- 16:00 End of the day 2
Thursday, 30 August 2018
the polymerase chain reaction
- 08:30 Theory: Introduction to PCR
- 09:30 Hands-On Laboratory: Qualitative PCR setup
- 10:00 Coffee break
- 11:00 Theory: PCR for GMO testing: Definitions and practices
- 12:00 Lunch
- 13:30 Theory: Real-time PCR for GMO quantitation
- 14:30 Hands-On Laboratory: Real-time PCR setup (in parallel – tubes, plates, and GenBit matrix approach)
- 15:00 Coffee break
- 15:30 Hands-On Laboratory: PCR results visualization and documentation
- 17:00 End of day 3
Friday, 31 August 2018
laboratory best practices
- 09:00 Theory: Protein based methods
- 10:00 Theory: ISTA rules for GMO detection
- 11:00 Theory: GMOs analysis in Russia, an update
- 12:00 Lunch
- 13:00 Assay and Process validation
- 14:00 Theory/Hands-On Laboratory: Data analysis and interpretation
- 14:30 Coffee break
- 15:00 Theory & discussion: Results analysis continued
- Stacked traits new challenges and solutions. Management and practices unique to the GMO testing lab
- 16:30 Conclusion
- 08:30 Opening and introductions
- 09:00 Introduction to GMO testing & workshop overview
- 10:00 Coffee break
testing plans
- 10:30 Theory: Testing plans I. Basic statistical concepts
- 11:15 Qualitative testing plans – Introduction to SeedCalc
- 12:00 Lunch
- 13:00 Theory: Testing plans II. Quantitative testing plans
- 14:30 Computer exercises
- 16:00 End of the day 1
Wednesday, 29 August 2018
from seed to dna
- 08:30 Theory: Sample Preparation: I. DNA Extractions
- 09:30 Introduction to the Hands-On laboratory procedure and design
- 10:00 Coffee break
- 10:30 Hands-On Laboratory: DNA extraction
- 12:00 Lunch
- 13:00 Theory: Sample Preparation: II. DNA quantitation, normalization, and sample tracking
- 14:30 Hands-On Laboratory: DNA visualization, quantitation and normalization
- 16:00 End of the day 2
Thursday, 30 August 2018
the polymerase chain reaction
- 08:30 Theory: Introduction to PCR
- 09:30 Hands-On Laboratory: Qualitative PCR setup
- 10:00 Coffee break
- 11:00 Theory: PCR for GMO testing: Definitions and practices
- 12:00 Lunch
- 13:30 Theory: Real-time PCR for GMO quantitation
- 14:30 Hands-On Laboratory: Real-time PCR setup (in parallel – tubes, plates, and GenBit matrix approach)
- 15:00 Coffee break
- 15:30 Hands-On Laboratory: PCR results visualization and documentation
- 17:00 End of day 3
Friday, 31 August 2018
laboratory best practices
- 09:00 Theory: Protein based methods
- 10:00 Theory: ISTA rules for GMO detection
- 11:00 Theory: GMOs analysis in Russia, an update
- 12:00 Lunch
- 13:00 Assay and Process validation
- 14:00 Theory/Hands-On Laboratory: Data analysis and interpretation
- 14:30 Coffee break
- 15:00 Theory & discussion: Results analysis continued
- Stacked traits new challenges and solutions. Management and practices unique to the GMO testing lab
- 16:30 Conclusion
from seed to dna
the polymerase chain reaction
- 08:30 Theory: Introduction to PCR
- 09:30 Hands-On Laboratory: Qualitative PCR setup
- 10:00 Coffee break
- 11:00 Theory: PCR for GMO testing: Definitions and practices
- 12:00 Lunch
- 13:30 Theory: Real-time PCR for GMO quantitation
- 14:30 Hands-On Laboratory: Real-time PCR setup (in parallel – tubes, plates, and GenBit matrix approach)
- 15:00 Coffee break
- 15:30 Hands-On Laboratory: PCR results visualization and documentation
- 17:00 End of day 3
Friday, 31 August 2018
laboratory best practices
- 09:00 Theory: Protein based methods
- 10:00 Theory: ISTA rules for GMO detection
- 11:00 Theory: GMOs analysis in Russia, an update
- 12:00 Lunch
- 13:00 Assay and Process validation
- 14:00 Theory/Hands-On Laboratory: Data analysis and interpretation
- 14:30 Coffee break
- 15:00 Theory & discussion: Results analysis continued
- Stacked traits new challenges and solutions. Management and practices unique to the GMO testing lab
- 16:30 Conclusion
laboratory best practices
You can launch questions to:
ISTA
FBSE "Rosselhoztsentr"
"GenBit" LLC