Validation of GenBit's matrix approach
    Reference samples

    Reference samples and their mixtures used for validation of the method of detection and identification of genetically modified organisms of plant origin with real-time PCR in matrix format on pre-filled matrix (GenBit) were provided by the FGBUN "Federal Research Center for Nutrition and Biotechnology". Certified Reference Materials (CRM) produced by ERM JRC EC, American Oil Chemists' Society and Sigma-Aldrich were used as well.  See also "test-systems"

    1. Mixtures of GM soybean lines in soybean flour
    1A
    line content (%)
    A2704-12 1.0
    A5547-127 0.5
    SYHT0H2 0.1
    MON87701 0.5
    1B
    line content (%)
    GTS 40-3-2 0.5
    MON89788 0.1
    FG72 1.0
    BPS-CV-127 0.5
    2. Mixtures of GM soybean lines in soybean flour
    2A
    line content (%)
    NK-603 0.1
    MON863 1.0
    GA21 0.5
    MON810 0.5
    2B
    line content (%)
    MON88017 0.5
    MON89034 1.0
    T25 0.5
    TC1507 0.1
    5307 0.1
    2C
    line content (%)
    3272 0.1
    BT11 0.5
    MIR604 1.0
    MIR162 0.5
    176* 0.1

     * line not approved in the Russian Federation
    3. Mixtures of GM crops lines in combined non-GMO maize, rice, soybean and wheat flour
    3A
    line content (%)
    LLRICE62 1.0
    H7-1 1.0
    GT73 (RT73)* 0.5
    BPS-CV-127 0.5
    GTS 40-3-2 0.1
    MON810 0.1
    176* 0.5
    3B
    line content (%)
    A2704-12 0.1
    A5547-127 0.5
    BT11 0.5
    3272 1.0
    FG72 0.5
    MON89034 0.1
    T25 0.5
    TC1507 1.0
    3C
    line content (%)
    MON89788 1.0
    SYHT0H2 0.5
    MON87701 0.1
    MIR604 0.1
    MON88017 0.5
    MON863 0.1
    NK603 1.0
    GA21 0.5
    5307 1.0
    MIR162 0.5
    3D
    line content (%)
    BPS-CV127 1.0
    MON87701 0.5
    MON89788 1.0
    FG72 1.0
    TC1507 0.5

     * line not approved in the Russian Federation
    Validation (examples)

    Sensitivity, linearity of the method

    click images to enlarge
    Miaize line LY038, dilutions
    DIlutions of LY038 maize line in non-GMO maize (starting 2 cernels of LY038 + 748 cernels of non-GMO maize).
    PCR-matrix "GMO: maize"
    Soybean line SYHT0H2, dilutions - linearity
    DIlutions of MON89788 soybean line in non-GMO soybean flour (0.1% - 10%).
    PCR-matrix "GMO: soybean"

    Matrix testing of 3A and 3C samples. Mixtures of GM-lines of agricultural crops in combined flour of non-GMO maize, rice, soybean and wheat. PCR matrix "GMO: screening"

    Simultaneous identification of transformation events

    Sample 3A - transformation events
    Sample 3C transformation events

    Simultaneous detection of genetic elements

    Sample 3A genetic elements
    Sample 3C genetic elements